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The Znu system contributes to growth during nutrient limitation imposed by the host protein calprotectin. WT, ΔznuA, ΔznuB, ΔznuC, and ΔznuD strains were grown for 6 h in 150 μg/ml recombinant human calprotectin, and OD600 was measured to determine growth compared to untreated. ****, P < 0.0001 by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple-comparison test. Data shown in biological triplicate as mean ± SD.
