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. 2019 Nov 18;87(12):e00732-19. doi: 10.1128/IAI.00732-19

FIG 2.

FIG 2

S. japonicum infection can induce NLRP3-inflammasome-dependent pyroptosis. Wild-type and NLRP3−/− mice were percutaneously treated with or without 30 ± 2 S. japonicum cercaria. At 6 weeks postinfection, liver tissue and serum were collected to analyze pyroptosis-associated protein and proinflammatory cytokine levels. (A) Western blot analysis of NLRP3, caspase-1, and IL-1β protein levels in liver. Con, control group; Inf, infected group; NLRP3−/−, NLRP3 deficiency group; NLRP3−/−+Inf, NLRP3−/− infected group. (B) ELISA of serum levels of IL-1β. (C and D) Quantification of IL-6 (C) and TNF-α (D) mRNA levels. (E) Serum levels of LDH measured with a biochemical analyzer. (F) Western blot analysis of GSDMD protein levels in liver. Data are mean ± SEM of 6 mice/group. **, P < 0.01 versus control; #, P < 0.05 versus infected; ##, P < 0.01 versus infected.