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. 2019 Nov 20;5(11):eaaw4717. doi: 10.1126/sciadv.aaw4717

Fig. 2. NPR-8-regulated collagen genes are required for C. elegans defense against P. aeruginosa infection.

Fig. 2

(A) npr-8(ok1439) and (B) WT animals grown on dsRNA for collagen genes or EV control were exposed to P. aeruginosa and scored for survival over time. The graphs are the combined results of three independent experiments. Each experiment included n = 60 adult animals per strain. P values in (A) are relative to npr-8 + EV: npr-8 + col-80 RNAi, P < 0.0001; npr-8 + col-93 RNAi, P = 0.0030; npr-8 + col-98 RNAi, P < 0.0001; npr-8 + col-101 RNAi, P < 0.0001; npr-8 + col-103 RNAi, P = 0.0601; npr-8 + col-160 RNAi, P = 0.0333; npr-8 + col-179 RNAi, P < 0.0001. P values in (B) are relative to WT + EV: WT + col-80 RNAi, P = 0.2555; WT + col-93 RNAi, P = 0.5575; WT + col-98 RNAi, P = 0.5250; WT + col-101 RNAi, P < 0.0001; WT + col-103 RNAi, P = 0.6950; WT + col-160 RNAi, P = 0.0494; WT + col-179 RNAi, P = 0.0004. (C) WT, npr-8(ok1439), col-8(sun1), and npr-8;col-80 animals were exposed to P. aeruginosa and scored for survival over time. P values are as follows: WT versus npr-8(ok1439), P < 0.0001; WT versus col-80(sun1), P = 0.8761; npr-8(ok139) versus npr-8;col-80, P < 0.0001. (D) WT, npr-8(ok1439), col-98(sun2), and npr-8;col-98 animals were exposed to P. aeruginosa and scored for survival over time. P values are as follows: WT versus npr-8(ok1439), P < 0.0001; WT versus col-98(sun2), P = 0.7044; npr-8(ok139) versus npr-8;col-98, P < 0.0001. (E) WT, npr-8(ok1439), col-179(ok3010), and npr-8;col-179 animals were exposed to P. aeruginosa and scored for survival over time. P values are as follows: WT versus npr-8(ok1439), P < 0.0001; WT versus col-179(ok3010), P < 0.0001; npr-8(ok139) versus npr-8;col-179, P < 0.0001.