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Exposure to simulated mucosal environments makes Flavobacterium columnare susceptible to phage infection. (A) FCL-2 titers over time on 0.5× Shieh cultures supplemented with primary rainbow trout mucus. (B) FCL-2 titers over time on 0.5× Shieh cultures supplemented with purified porcine mucin. Each data point represents the average of triplicates and their standard deviation. Unpaired t tests were used to compare the controls and tested conditions (*, P < 0.05; **, P < 0.001).
