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. 2019 Nov 19;10(6):e02289-19. doi: 10.1128/mBio.02289-19

FIG 5.

FIG 5

Se and Zn limitation decrease C. difficile proline fermentation. (A) 5-Aminovalerate concentrations in spent medium of C. difficile grown in the presence of 75 μM TPEN. TY was supplemented with l-proline (30 mM final concentration). Each dot represents a single replicate. Bacteria were grown to early exponential phase (OD600 of ∼0.2) (B) The intracellular NAD+/NADH ratios of WT or prdB::CT C. difficile grown in TY medium with or without 75 μM TPEN or 75 μM ZnCl2. (C) Intracellular selenium abundance in C. difficile grown in TY medium with or without 75 μM TPEN or 10 μM Na2SeO3 determined through ICP-MS. (D) 5-Aminovalerate concentrations in spent medium from C. difficile grown in TY medium with or without 75 μM TPEN, 10 μM Na2SeO3, or 75 μM ZnCl2 at the indicated time points. Error bars indicate standard deviations. (E) PrdB peptide (TAVIVQR) abundance relative to abundance of an SlpA S-layer peptide (LYNLVNTQLDK). The PrdB peptide is downstream of the selenocysteine residue. Data represent 2 to 3 replicates. Error bars indicate standard deviations. *, P < 0.05 by unpaired t test.