TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant information | Reference or source |
|---|---|---|
| Strains | ||
| Clostridioides difficile strain R20291 | Ribotype 027 | 33 |
| C. difficile prdR::CT | ClosTron intron inserted after nucleotide 391 in prdR (prdR 391a::CT), Lincr | This study |
| C. difficile prdB::CT | ClosTron intron inserted after nucleotide 556 in prdB (prdB 556a::CT), Lincr | This study |
| Bacillus subtilis JH BS2 | Tn196, TetR | 64 |
| Escherichia coli DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) λ2212 | 64 |
| E. coli MG1655 | RecA+ | 63 |
| Plasmids | 64 | |
| pCR-Blunt | Cloning vector, Kanr | Thermo Fisher |
| pJS107 | Tn916, oriT | 64 |
| pCAL127 | pJS107_prdR intron | This study |
| pCAL128 | pJS107_prdB intron | This study |
| pJS116 | pCD6 ColE1 Tn916 oriT, Cmr | 64 |