FIG 3.

The E. faecalis protease GelE degrades EHEC EspB. (A) EspB protein levels from EHEC cultures with or without E. faecalis measured by dot blot assay. Intensity of the spots was measured using ImageJ and converted to absolute EspB concentration using a standard curve of known recombinant EspB concentrations included on the same blot. The average and standard deviation of three replicates from one experiment are plotted here, and the experiment was repeated three times. ND, not detected (insufficient signal). (B) EspB protein levels from EHEC cultures with or without E. faecalis measured by ELISA. EspB concentrations in samples were calibrated to a standard curve of known recombinant EspB concentrations. The average and standard deviation of three technical replicates are plotted here, and the experiment was repeated twice. NT, not tested. Two-tailed t tests were performed to determine significant differences compared to results for EHEC alone. *, P < 0.05; **, P < 0.01; ***, P < 0.001.