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The effects of lipid supplementation on the ali1Δ mutant. Serial dilutions of the WT, ali1Δ mutant, and ali1Δ + ALI1 strains were incubated on YPD medium; YPD with caffeine (1 mg/ml); YPD with caffeine, ox bile (10 mg/ml), and Tween 60 (1%); and YPD with caffeine and glycerol (0.4%). (A and B) These strains were incubated at 30°C (A) and 37°C (B) and monitored visually for growth.
