TABLE 4.
Plasmids used in this study
| Plasmid | ORFa | Backbone | Reference or source |
|---|---|---|---|
| pJAF | Neomycin resistance cassette (NEO) | 95 | |
| pCH233 | Nourseothricin resistance cassette (NAT) | 96 | |
| pCT1 | ALI1 (including promoter and terminator) NAT | pCH233 | This study |
| pCN20 | Histone H3 promoter, NAT | pCH233 | 97 |
| pKS85 | Histone H3 promoter, RRA1 GFP RRA1 (terminator only) NAT | pCH233 | 26 |
| pCT3 | Histone H3 promoter, ALI1 GFP RRA1 (terminator only) NAT | pUC19 | This study |
| pCT11 | ALI2 (including promoter) GFP FKS1 (terminator only) NEO | pUC19 | This study |
| pCT8 | ALI3 (including promoter and terminator) NEO | pJAF | This study |
| pCT10 | ALI4 (including promoter and terminator) NEO | pJAF | This study |
a
ORF, open reading frame.