FIG 4.

(A) Heat map showing the variance stabilizing transformation-normalized read counts of S. aureus in SCFM2 at mid-logarithmic phase or SCFM2 plus HNP-1 at mid-logarithmic phase or in human sputum for virulence factors known to be directly regulated by the SaeRS two-component system (47). Blue indicates higher read counts. (B) Differential gene expression of S. aureus virulence factors from transcriptomes grown in SCFM2 at mid-logarithmic phase or SCFM2 plus HNP-1 at mid-logarithmic phase compared to transcriptomes from human sputum. Categories that shifted with the addition of HNP-1 are highlighted in blue. The percent differentially expressed genes (>2-fold change in expression, adjusted P value of <0.05) in each category was calculated. SCFM2 experiments were performed at mid-logarithmic phase.