TABLE 3.
Thirty-two genes that comprise a transcriptional signature that can reliably distinguish between S. aureus transcriptomes from in vitro growth and those from human CF sputum
| Locus tag | Gene | Functiona |
|---|---|---|
| SAUSA300_RS00540 | AraC family transcriptional regulator | |
| SAUSA300_RS00605 | sirA | Iron ABC transporter substrate-binding protein |
| SAUSA300_RS00650 | sbnI | Siderophore biosynthesis protein |
| SAUSA300_RS01135 | uhpT | Hexose phosphate transporter |
| SAUSA300_RS01250 | ldh1 | l-Lactate dehydrogenase |
| SAUSA300_RS01655 | PTS sugar transporter subunit IIC | |
| SAUSA300_RS02160 | ssl9 | Staphylococcal superantigen-like protein 9 |
| SAUSA300_RS02600 | Hypothetical protein | |
| SAUSA300_RS02770 | 23S rRNA [guanosine(2251)-2'-O]-methyltransferase | |
| SAUSA300_RS03050 | eutD | Phosphate acetyltransferase |
| SAUSA300_RS03080 | Dihydrolipoamide dehydrogenase | |
| SAUSA300_RS03585 | Hypothetical protein | |
| SAUSA300_RS04015 | lgt | Prolipoprotein diacylglyceryl transferase |
| SAUSA300_RS04025 | Putative PEP-CTERM system TPR-repeat lipoprotein | |
| SAUSA300_RS04580 | Pyridine nucleotide-disulfide oxidoreductase | |
| SAUSA300_RS05430 | typA | Translational GTPase |
| SAUSA300_RS05545 | isdC | Iron-regulated surface determinant protein C |
| SAUSA300_RS05555 | isdE | Heme uptake system protein |
| SAUSA300_RS06485 | glnA | Glutamine synthetase |
| SAUSA300_RS07355 | bshA | N-Acetyl-alpha-d-glucosaminyl l-malate synthase |
| SAUSA300_RS07900 | xerD | Tyrosine recombinase |
| SAUSA300_RS07905 | fur | Ferric uptake regulator |
| SAUSA300_RS09130 | ptaA | PTS glucose transporter subunit IIBC |
| SAUSA300_RS11445 | fbaA | Fructose-bisphosphate aldolase |
| SAUSA300_RS11760 | htsA | ABC transporter substrate-binding protein |
| SAUSA300_RS12210 | AcrB/AcrD/AcrF family protein | |
| SAUSA300_RS12780 | lctP2 | l-Lactate permease |
| SAUSA300_RS13075 | hlgC | Gamma-hemolysin component C |
| SAUSA300_RS13330 | cntF | ABC transporter ATP-binding protein |
| SAUSA300_RS13355 | cntM | Staphylopine synthase |
| SAUSA300_RS13745 | cidC | Pyruvate oxidase |
| SAUSA300_RS14605 | DNA-binding protein |
a
Abbreviations: PTS, phosphotransferase system; PEP, proline-glutamate-proline; CTERM, C terminal; TPR, tetratricopeptide repeats.