2.

Dosage and administration methods of indocyanine green

Purpose	Injection time	Administration method	Injection dosage
IV: Injection through peripheral vein; NAH: Nonanatomical hepatectomy; AH: Anatomical hepatectomy; PV: Injection through portal vein of the targeted hepatic segment; IA: Injection through femoral artery puncture surpassing selective hepatic segment artery.
Identification and location of tumors	Based on ICG R15	IV	0.25-0.5 mg/kg
Definition of tumor boundaries	Based on ICG R15	IV	0.25-0.5 mg/kg
Detection of residual liver	Based on ICG R15	IV	0.25-0.5 mg/kg
Hepatic segmentation (Negative Display Method)	Intraoperative	IV	1 mL (2.5 mg/mL)
Hepatic segmentation (Positive Display Method)	Intraoperative	PV	0.1 mL (2.5 mg/mL) mixed with 5.0 mL indigo carmine
Hepatic segmentation (Experimental phase)	Intraoperative	IA	1 mL (1 mg/mL)
Delineation of hepatectomy line (NAH)	Preoperative	IV	0.25-0.5 mg/kg
Delineation of hepatectomy line (AH)	Intraoperative	IV/PV	Refer to hepatic segmentation dose
Detection of Biliary Leakage in Liver Section	Intraoperative	Transcystic duct	5-10 mL (2.5 mg/mL)
Living donor liver transplantation (Biliary reconstruction)	Intraoperative	Transcystic duct	2 mL (2.5 mg/mL)
Living donor liver transplantation (Revascularization)	Intraoperative	IV	1.5 mL (2.5 mg/mL)