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MASCOT 2.6数据库搜索相关参数

Related parameters for MASCOT 2.6 database searching

Parameter	Value
Protein database	Uniprot_HomoSapiens_20386_20180905
Enzyme	Trypsin
Max missed cleavages	2
Instrument	ESI-TRAP
Precursor mass toleranc	±10 ppm
Fragment mass tolerance	0.05 Da
Use average precursor mass	False
Modification groups from quan method	TMT 6plex
Dynamic modifications	Oxidation（M）Acetyl（Protein N-term）
Static modifications	Carbamidomethyl（C）
Database pattern	Decoy
Peptide FDR	≤0.01