FIGURE 6.

Evans blue inhibits the infection of drug-resistant strains of HBV. Huh7 cells transfected with an HBV plasmid carrying drug resistance mutation(s) as described in Section “Materials and Methods” were cultured in DMEM media for 4 days. The supernatant virions were collected and used to infect Huh7D^hNTCP cells at Geq = 2000. Six days after infection, total HBV DNAs were extracted for PCR analysis. Drug-resistant mutations P117G (A) and F249A (B) are tenofovir resistant, N236T (C) is adefovir resistant, and L180M + M204V + S202G is 3TC/ETV-dual-resistant (D). Statistical significance was determined using Two tails Student’s t-test (^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001).