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. 2019 Nov 14;7:279. doi: 10.3389/fcell.2019.00279

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Copyright © 2019 Lawrimore, Doshi, Walker and Bloom.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleolar model organization and simulated fluorescent images. (A–C) Volumetric model of the budding yeast nucleolus with differing bead-bead dynamic crosslinking durations. Red beads represent rDNA locus. The green banded sphere represents the nuclear membrane which acts as a boundary constraint in the simulation. (D–F) Simulated fluorescent images of the nucleolar models. The parameter μ indicates the average duration of a bead-bead crosslink.