TABLE 1.
Features of interest ranked in order of their importance by PCA.
| Feature | Rank | Description |
| Kinetochore distance to SPB | 1 | Euclidean distance between the brightest pixel of the kinetochore and the brightest pixel of its closest SPB foci normalized by the length of the spindle |
| Kinetochore X-distance | 2 | Magnitude of the distance between the brightest pixel of the kinetochore and the brightest pixel of its closest SPB foci parallel to the spindle axis, normalized by the length of the spindle |
| SPB foci mean intensity | 3 | Mean intensity of a 5 × 5 region encompassing the SPB foci |
| SPB foci height | 4 | Full-width-half-max of the maximum projection of the 7 × 15 region about the brightest pixel of the SPB foci perpendicular to the spindle |
| SPB foci STD in Y | 5 | Standard deviation of the intensities generated from a line-scan of a SPB foci perpendicular to the spindle |
| SBP foci STD in X | 6 | Standard deviation of the intensities generated from a line-scan of a SPB foci parallel to the spindle |
| SPB Foci STD | 7 | Standard deviation of intensities of 5 × 5 region encompassing the SPB foci |
| Kinetochore foci height | 8 | Full-width-half-max of the maximum projection of the 7 × 15 region about the brightest pixel of the kinetochore foci perpendicular to the spindle |
| Kinetochore foci STD | 9 | Standard deviation of intensities of 5 × 5 region encompassing the kinetochore foci |
| Kinetochore foci STD in Y | 10 | Standard deviation of the intensities generated from a line-scan of a SPB foci parallel to the spindle |
| K–K distance | 11 | Euclidean distance between the brightest pixels of the two kinetochore foci, normalized by the length of the spindle axis |
| Kinetochore foci mean intensity | 12 | Mean intensity of a 5 × 5 region encompassing the kinetochore foci |
| Kinetochore foci STD in X | 13 | Standard deviation of the intensities generated from a line-scan of a kinetochore foci parallel to the spindle |