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. 2019 Nov 20;9:17160. doi: 10.1038/s41598-019-53663-8

Figure 1.

Figure 1

Overview of the DL-MSS method. Automatic tracking software is used to obtain single molecule trajectories from fluorescence microscopy data. A trained deep learning (DL) neural network is applied to these trajectories to segment them into “tracklets” of consecutive track points that were classified to have the same type of mobility. Tracklets are further analyzed using the moment scaling spectrum (MSS) to acquire the properties associated with each class.