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. 2019 Nov 20;9:17220. doi: 10.1038/s41598-019-53572-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Screen with unbiased genome-wide CRISPR knockout library in MEF-LoxP-KC cells. (A) Schematic of the first unbiased genome-wide CRISPR knockout. (B) Representative colonies were identified in soft agar 3 weeks after transduced MEFs were seeded in soft agar. (C) The list of sgRNAs which were identified in colonies from the soft agar screen.