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. 2019 Nov 20;9:17220. doi: 10.1038/s41598-019-53572-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Individual validation of candidate genes that suppress transformation from the screen with genome-wide CRISPR sgRNA library without Trp53 sgRNAs. (A) MEF-LoxP-KC cells transduced with a single candidate sgRNA were plated in soft agar or injected into the muscle of nude mice (n = 2 or 3). (B) MEF-LoxP-KC cells transduced with more than one sgRNA targeting Slc9a3 were plated in soft agar assay or injected into the muscle of nude mice (n = 2 or 3). The result is representative of at least two independent experiments. Scale bars = 100 µm.