Fig. 4.
EC-specific knockout of Parva phenocopies IlkiECKO vascular defects. a Confocal images of isolectin B4 stained P16 control and ParvaiECKO retinas. Note large and blind-ended EC bulges in the ParvaiECKO but not the control vasculature. Scale bar, 150 μm (top), 50 μm (center), and 25 µm (bottom). b, c Images of isolectin B4-stained retinal vessels with pseudo-coloring of the NFL (green) and OPL (red) vasculature (b). ParvaiECKO EC bulges are confined to the NFL, whereas the mutant OPL vessel density is decreased resulting in a significant increase of the NFL/OPL EC area ratio (c). Scale bar, 50 μm in (b). Error bars, s.e.m. p values (***p < 0.001), Student’s t test (n = 5 control and 4 mutant retinas). d XZ projection images of isolectin B4-stained control and Parva iECKO retinas. Mutant NFL vessels are enlarged and vertical sprouting is impaired. Scale bar, 25 μm. Source data are provided as a Source Data file