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. 2019 Nov 20;9:17171. doi: 10.1038/s41598-019-53665-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of endogenous PAK localization by immunofluorescence staining. (a) PAK2 antibody specificity control. The signal from PAK2 antibody, combined with an AlexaFluor647-conjugated secondary antibody, was compared with the eGFP signal in HeLa cells transfected with PAK2-eGFP. (b) Top: HeLa cell-surface adhesion points visualized by interference reflection microscopy (IRM) in untransfected cells. Bottom: PAK2 signal (green) is superposed on IRM signal from the same visual field. The scale bars correspond to 10 µm.