Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 20;2:422. doi: 10.1038/s42003-019-0663-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — MamoAF overexpression induces vas expression in embryos. a Developmental western blotting analysis of embryos expressing full-length Mamo-FLAG under the control of matα4-Gal-VP16 driver. Embryos were cultured at 18 °C for indicated hours and subjected to western blotting with anti-FLAG antibody. Embryos cultured at 18 °C for 0, 6, 16 and 24 h are corresponded to embryonic stage 4, 9, 12 and 15, respectively. b Analysis of fractionated samples extracted from embryos expressing full-length Mamo-FLAG by western blotting. Embryos expressing Mamo-FLAG under the control of matα4-Gal-VP16 driver were cultured at 25 °C for 0–16 h and fractionated. Normalised load of each extract (7 μg) were analysed by western blotting with anti-FLAG, anti-Tubulin and anti-Histone H3 antibodies. Whole extract (lane 1), cytoplasmic extract (lane 2) and nuclear extract (lane 3) were loaded. c Analysis of fractionated samples extracted from embryos expressing MamoAF-FLAG by western blotting. Embryos expressing MamoAF-FLAG under the control of mat4α-Gal-VP16 driver were cultured at 25 °C for 0–16 h and fractionated. Normalised load of each extract (11 μg) were analysed by western blotting with anti-FLAG, anti-Tubulin and anti-Histone H3 antibodies. Whole extract (lane 1), cytoplasmic extract (lane 2) and nuclear extract (lane 3) were loaded. Full images of the electrophoreses are shown in Supplementary Fig. 16. d qRT-PCR analyses of Vas mRNA in wild type, Mamo and MamoAF overexpressed embryos under the control of matα4-Gal-VP16 driver. Error bars are SD of the mean. **P < 0.01, *P < 0.05 (two-tailed Student’s t test). e Double fluorescence in situ hybridisation for Vas and Elav mRNAs of wild type, the embryos overexpressing Mamo, MamoAF and MamoAF fragments under the control of matα4-Gal-VP16 driver. Elav is a neural marker. Confocal sections of the brain of the embryos are shown. The inset shows close-up image of wild-type PGCs. f Double-staining for Vas and neural marker Elav of wild type, the embryos overexpressing Mamo, MamoAF and MamoAF fragments under the control of matα4-Gal-VP16 driver. Arrows indicate PGCs expressing Vas. Arrowheads indicate ectopic Vas expression. Scale bar: 30 μm