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. 2019 Nov 20;2:422. doi: 10.1038/s42003-019-0663-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — MamoAF collaborates with OvoB to induced Vas expression in brain. a Immunostaining of embryos carrying OvoB-Nterm-egfp allele and MamoAF OE carrying OvoB-Nterm-egfp allele showing MamoAF induces ectopic GFP expression from the OvoB-Nterm-egfp allele. MamoAF is overexpressed under the control of maternal matα4-Gal-VP16 driver. Scale bar: 50 μm. b Wild type, MamoAF OE, OvoA OE, OvoB OE, OvoA + MamoAF OE, OvoB + MamoAF OE, OvoA + OvoB OE and OvoA + OvoB + MamoAF OE are stained for Vas, Elav and DAPI. The transgenes are overexpressed under the control of maternal matα4-Gal-VP16 driver. Whole embryos are shown in left, close-up views of brain of the embryos are in right panels. Scale bar: 50 μm