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. 2019 Nov 20;2:422. doi: 10.1038/s42003-019-0663-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — MamoAF collaborates with OvoB to induce Vas expression in PGCs. a Vas mRNA in situ hybridisation of wild type, MamoAF overexpressed and MamoAF and OvoA overexpressed embryos under the control of maternal nos-Gal4 driver at stage 14. White arrowheads indicate embryonic gonads. b Embryos were classified into three groups depending on their strong (++), middle (+) and low (−) signal intensities of Vas mRNA in PGCs. Percentages of the embryos carrying PGCs with strong, middle and low signals in wild type, MamoAF-OE and MamoAF + OvoA OE embryos. **P < 0.01 (Significance is calculated with MamoAF OE by Fisher’s exact test). c Vas mRNA in situ hybridisation of wild type and OvoB overexpressed embryos under the control of maternal nos-Gal4 driver at stage 15. White arrowheads indicate embryonic gonads. d Embryos were classified into three groups depending on their strong (++), middle (+) and low (−) signal intensities of Vas mRNA in PGCs. Percentages of the embryos carrying PGCs with strong, middle and low signals in wild type and OvoB-OE embryos. *P < 0.05 (Significance is calculated with wild type by Fisher’s exact test). Scale bar: 20 μm