Fig. 6.

MamoAF and OvoB exhibit transcriptional synergy. a vas-A element and the Ovo-binding consensus sequences (Ovo1 and Ovo2) in the first intron of vas. b–e Luciferase reporter assays in S2 cells, showing that both Ovo1 and vas-A element are required for transcriptional activation of luciferase. All experiments were conducted as triplicate and the mean of relative luciferase activities and SD are shown. *P < 0.05, **P < 0.01 (two-tailed Student’s t test). f Luciferase reporter assays in S2 cells, showing that MamoAF, OvoB and CBP collectively stimulates luciferase expression. **P < 0.01 (two-tailed Student’s t test). All experiments were conducted as triplicate and the mean of relative luciferase activities and SD are shown. g Coimmunoprecipitation assays with nuclear extracts of S2 cells that had been transfected with both FLAG-tagged MamoAF and V5-tagged OvoB. The precipitate with a V5 antibody contains not only OvoB but also MamoAF-FLAG, showing MamoAF physically interacts with OvoB. Full images of the electrophoreses are shown in Supplementary Fig. 16. h A model of interaction between MamoAF, CBP and OvoB in the activation of vas expression