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. 2019 Nov 20;9:17197. doi: 10.1038/s41598-019-53722-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA digestion and confirmation by the optical map. (A) Schematic of XbaI digested λ DNA patterned with tTALE-FP (2 nM) to reveal the genome-specific pattern (made in ©BioRender - biorender.com). (B) λ DNA in a mixture of tTALE-FP and NaCl solution. Yellow arrows indicate whole λ DNA and pink arrows indicate digested λ DNA, compared with AT-specific consensus genomic map.