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. 2019 Nov 20;9:17195. doi: 10.1038/s41598-019-53900-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Marker gene analysis of XY gonad development in Adamts16 homozygous mutants. Expression of the Sertoli cell marker Sox9 (A), Leydig cell marker Insl3 (B) and the germ cell meiotic entry marker Stra8 are all unaffected in Adamts16 homozygous mutants (−/−) when compared to wild-type (+/+) and heterozygous (+/−) controls at 14.5 dpc. Scale bar = 0.5 mm.