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. 2019 Nov 20;9:17127. doi: 10.1038/s41598-019-53714-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histopathology of the lung tissues of mice at 4 (A), 24 (B), and 48 h (C) after inoculation with A. baumannii (H&E; bar = 100 µm). (A) At 4 h after inoculation, no significant lesion was present in the alveoli, bronchioles, or bronchi, except for minimal edema in the alveolar cavity. (B) At 24 h after inoculation, moderate numbers of neutrophils (dark triangle) and macrophages (blue triangle) had infiltrated the alveoli; moderate edema was present, and bacterial colonization (dark arrow) was observed in the alveolar cavities. (C) At 48 h after inoculation, large numbers of neutrophils (dark triangle) and macrophages (blue triangle) had infiltrated the alveoli.