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. 2019 Nov 20;9:17210. doi: 10.1038/s41598-019-53218-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PXR is present in human platelets. (A) PXR was immunoprecipitated (IP: PXR) from human platelets (IP: PXR) using a mouse monoclonal antibody and blotted with a rabbit polyclonal antibody. Human whole platelet lysates (WPL) and antibody used to IP (Ab: PXR) PXR were used as positive and negative controls, respectively. Cropped western blot image is representative of 3 separate experiments using different donors. Full length blot is shown in Supplementary Fig. 8a (B) The localisation of PXR in human resting platelets was investigated using immunofluorescence microscopy. PXR (in red) and membrane GPIb receptors (in green) were stained using anti-PXR and anti-GPIb antibodies. Platelets without primary antibody treatment were used as negative controls. (C) Flow cytometry was used to examine the median fluorescence level of PXR in permeabilised resting and activated (with 0.1 U/ml thrombin) human platelets, incubated with a PXR antibody or the equivalent rabbit IgG isotype control. (D) Median fluorescence intensity (MFI) associated with the PXR positive platelets in resting and activated platelets. Data represent mean ± SD (N ≥ 3), *P < 0.05 was calculated by Student T-test. Figure adapted from corresponding PhD thesis⁴⁸.