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. 2019 Nov 20;9:17210. doi: 10.1038/s41598-019-53218-x

Figure 5.

Figure 5

SR12813 inhibit thrombosis and haemostasis. Laser-induced injury model was used to measure thrombosis in hPXR mice through intravital microscopy. Vehicle-control (DMSO 0.1% v/v) or SR12813 (100 μM) was administered intravenously to mice and incubated for 20 minutes. Platelets were labelled with Alexa488–conjugated anti-GPIb antibody. (A) Representative images of thrombi obtained at different time intervals. (B) Data represent median fluorescence intensity, measured for 8 to 10 thrombi from 3 mice each of control and SR12813 treated groups. (C) Thrombus-size was calculated using area under the median fluorescence intensity curve of each thrombi. (D) Mean of maximum fluorescence intensity of the thrombus. (E) Tail bleeding was performed on hPXR mice pre-treated with vehicle or SR12813 (100 μM) for 20 min (n = 7). Data represent mean ± SD (n ≥ 3) where *P < 0.05, **P < 0.01 and ***P < 0.001 was determined by nonparametric Mann–Whitney U test. Figure adapted from corresponding PhD thesis48.