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. 2019 Nov 12;5:55. doi: 10.1038/s41421-019-0122-x

Fig. 1. The Asprosin-OLFR734 signaling axis promotes sperm progressive motility and enhances male fertility.

Fig. 1

a qPCR results showing relative mRNA levels of Olfr734 in different tissues from mice. Data are shown as mean ± s.e.m. n = 5 mice. b–d Sperm motility (b), two-cell states (c) and sperm ATP content (d) from 10-week-old WT and Olfr734−/− ad lib-fed mice. To calculate two-cell states, 10 WT female mice were mated to 5 male WT or Olfr734−/− mice (two female mice for one male mouse). NS, no significant statistical difference. Data are shown as mean ± s.e.m. *P < 0.05, ***P < 0.001, n = 5 mice. e, f Sperm progressive motility (e) and two-cell states (f) from 10-week-old or 40-week-old WT and Olfr734−/− mice. Mice were intraperitoneally injected with purified GST or GST-Asprosin (60 μg kg-1) for 10 days before isolation of sperm. To calculate two-cell states, 10 WT female mice were mated to 5 male WT or Olfr734−/− mice (two female mice for one male mouse). Data are shown as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, n = 5 mice. g, h ATP (g) and cAMP (h) content in sperm from 10-week-old or 40-week-old WT and Olfr734−/− mice. Mice were intraperitoneally injected with purified GST or GST-Asprosin (60 μg kg-1) for 10 days before isolation of sperm. Data are shown as mean ± s.e.m. *P < 0.05, **P < 0.01, n = 5 mice