Figure 1.

Western analysis of wild-type and variant CD36 expression. Cells were transfected with wild-type (WT) and variant CD36 constructs, as indicated. NonG is a non-glycosylatable CD36 derivative in which all ten putative glycosylation sites have been mutated. UT designates lysate from untransfected cells. CD36 expression was detected using mAb1955 (top panel). Anti-β-tubulin was used as loading control (bottom panel). Vertical dashed lines indicate the position of irrelevant lanes that were cropped from the Western blot. Full-length blots/gels are presented in Supplementary Fig. 1.