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. 2019 Nov 20;9:17213. doi: 10.1038/s41598-019-53610-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-leukemic, clinically healthy NP23-NHD13 mice are lymphopenic. (a–c) Flow cytometry of indicated tissues from young WT and NP23-NHD13 mice. (d) Absolute cell number of NP23-NHD13 thymus and thymic subsets (DN-double negative, CD4⁺, CD4⁺/CD8⁺ and CD8⁺) compared to WT. (e) Percentage of thymic subsets (DN-double negative, CD4⁺, CD4⁺/CD8⁺ and CD8⁺) compared to WT. Data are expressed as means ± SD, n = 3 mice per group. ns = not significant, *P < 0.05, ****P < 0.001, by one tailed student t-test.