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. 2019 Nov 20;9:17213. doi: 10.1038/s41598-019-53610-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thymic AML is transplantable. (a) Flow cytometry of thymus invaded by AML cells (#4369). (b) Survival of WT (total thymocytes) and NP23-NHD13 (bulk thymic AML cells) transplant recipients, 1 × 10⁶ cells /mouse, n = 5 mice per group. Data are analyzed by Log-rank (Mantel-Cox) test. (c) Engraftment of CD45.2⁺/Mac1⁺ cells at indicated time points (dotted circle at 4 wk time point indicates engraftment data from BM). (d) Flow cytometry of representative transplant recipient (R-4) shows engraftment of leukemic myeloid cells in both BM and thymus. (e) Quadruple negative (QN) thymocytes (Mac1⁻/Gr1⁻ and CD4⁻/CD8⁻, 8.4 × 10⁴ cells/mouse) transplanted from donor #4369, WT-QN thymocytes (9.6 × 10⁴ cells/mouse) are used as control, n = 5 mice per group. (f) Survival of WT and NP23-NHD13 (QN) transplant recipients analyzed by Log-rank (Mantel-Cox) test. (g) Engraftment of CD45.2⁺/Mac1⁺ cells at indicated time points (dotted circle at 4 wk time point indicates engraftment data from BM). (h) Flow cytometry of representative transplant recipient (R-2) indicates engraftment and myeloid commitment of the transplanted QN thymocytes in BM and thymus. **P < 0.01.