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. 2019 Nov 20;9:17213. doi: 10.1038/s41598-019-53610-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transplantation of QN thymocytes from non-leukemic NP23-NHD13 thymic cells leads to AML. (a) Purification of QN thymocytes from non-leukemic thymus and transplantation of 100 cells per recipient. (b) Survival curve of WT and NP23-NHD13 QN recipients, n = 4–5 mice per group. Data are analyzed by Log-rank (Mantel-Cox) test. c Engraftment of Ly45.2⁺/Mac1⁺ cells at indicated time interval (dotted circle indicates engraftment data obtained from BM). (d) Flow cytometry profile of representative transplant recipient (R-1) indicates engraftment and differentiation of QN thymocytes in both BM and thymus. ^**P < 0.01.