. 2019 Oct 4;12(10):1524–1528. doi: 10.14202/vetworld.2019.1524-1528

Table 1.

Uniplex PCR: Primers sequences, target operons, amplicon sizes, and cycling conditions.

Target operon	Primers sequences	Amplified segment (bp)	Primary denaturation	Amplification (35 cycles)			Final extension	Reference

				Secondary denaturation	Annealing	Extension
mex R	GCGCCATGGC CCATAT TCAG	637	94°C 5 min	94°C 45 s	57°C 45 s	72°C 45 s	72°C 10 min	[16]
mex R	GGCATTC GCC AGTAAGCGG	637	94°C 5 min	94°C 45 s	57°C 45 s	72°C 45 s	72°C 10 min	[16]

PCR=Polymerase chain reaction