Table 2.
Multiplex PCR: Primers sequences, target genes, amplicon sizes, and cycling conditions.
| Target gene | Primers sequences | Amplified segment (bp) | Primary denaturation | Amplification (30 cycles) | Final extension | Reference | ||
|---|---|---|---|---|---|---|---|---|
| Secondary denaturation | Annealing | Extension | ||||||
| exo S | CCTTCCCT CCTTCCCCC CGGCGATCTGGA AAAG AAATG | 270 | 95°C 5 min | 95°C 30 s | 58°C 30 s | 72°C 50 s | 72°C 10 min | [17] |
| CATCCTCA GGCG TACATCCT | ||||||||
| pcl H | GAAGCCAT GGGCT ACTTCAA | 307 | ||||||
| AGAGTGA CGAGG AGCGGTAG | ||||||||
| tox A | ATGGTGTA GATC GGCGACAT | 433 | ||||||
| AAGCCTTC GACC TCTGGAAC | ||||||||
PCR=Polymerase chain reaction