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. 2019 Nov 20;9:17188. doi: 10.1038/s41598-019-53659-4

Figure 2.

Figure 2

YAP associates with F-actin under activating conditions. MCF10A cells densely or sparsely plated on (a) 2D glass coverslips or (b) 2D col-1 coated PAM gels, YAP:F-actin analysis in last column indicates overlapping regions of ≥60% YAP pixel intensity and ≥60% F-actin pixel intensity. YAP (green), F-actin stained with phalloidin (red), DNA stained with DAPI (blue). Bar: 10 µm. (c) MCF10A cells encapsulated for seven days in soft and stiff IPNs and stained as above. Bar: 10 µm. (d) Table of YAP activation in relation to dimensionality and stress fiber formation. Grey boxes indicate conditions that activate YAP.