Table 2.
Comparison of expanded clones from controls and patients analyzed using the chi-squared test
| DQ6 tetramer | Controls expanded/total cells (% cells expanded) | Patients expanded/total cells (% cells expanded) | Chi-squared test (P-value) | FDR correction | |
|---|---|---|---|---|---|
| q-value | Discovery? (P < 0.047619) | ||||
| All | 134/2660 (5.04) | 117/2843 (4.12) | 0.1013 | 0.063819 | No |
| HCRT1–13 | 74/1141 (6.49) | 71/1143 (6.21) | 0.7884 | 0.41391 | No |
| HCRT25–37 | 2/384 (0.52) | 0/576 (0) | 0.0829 | 0.063819 | No |
| HCRT56–69 | 52/384 (13.54) | 20/384 (5.21) | <0.0001 | 0.000156 | Yes |
| HCRT87–100 | 6/751 (0.8) | 26/740 (3.51) | 0.0003 | 0.000315 | Yes |
| Excluding TIV-vaccinated subjects | |||||
| HCRT87–100 | 0/463 (0) | 24/644 (3.73) | <0.0001 | 0.000156 | Yes |
Statistics was corrected with the Benjamini, Krieger and Yekutieli false discovery rate (FDR)-controlling procedure, desired FDR (Q): 5%. q-value: adjusted P-value. Discovery indicates significant skewing of expanded clonotype detection in one group versus another. Analysis excluding TIV-vaccinated subject is performed on DQ6-HCRT87–100 tetramer-sorted cells