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. 2019 Nov 20;9:17152. doi: 10.1038/s41598-019-53676-3

Figure 2.

Figure 2

Relevance of trophoblast endogenous VIP for glucose uptake in Swan 71 and BeWo cells. Trophoblast cells (Swan 71 and BeWo cell lines) were grown as in Fig. 1. 2-NBDG was added for 10 min (a,c) or 3 min (b), cells were washed with cold PBS and flow cytometry was performed. Results are expressed as the difference between without/with 1 mM Phloretin. (a) 2-NBDG uptake of cells incubated with 50 nM VIP or 50 ng/ml LIF 10 min before the addition of 2-NBDG (n = 9 for both Swan 71 and BeWo cell lines in VIP treatment and n = 6 in LIF treatment). (b) Representative fluorescence microscopy of both cell lines after 3 min incubation with 2-NBDG without/with 50 nM VIP. (c) 2-NBDG uptake after VIP was knocked down for 72 h and 2-NBDG were added for 10 min (n = 4). ANOVA with Dunnett’s multiple comparisons against basal condition (a) or Student’s t-test was used (c). Results are expressed as Mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001.