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. Author manuscript; available in PMC: 2020 Nov 20.
Published in final edited form as: Bioconjug Chem. 2019 Oct 22;30(11):2947–2957. doi: 10.1021/acs.bioconjchem.9b00640

Figure 4. Confocal microscopy of fluorescently labeled NDs and ND-ProGlo.

Figure 4.

This data supports shows the difference in uptake mechanism observed with micropinocytosis inhibitor EIPA. In T47D cells treated with ND-ProGlo, fluorescence is primarily associated with the outside of the cell. Fluorescent NDs without ProGlo seem to undergo higher levels of cellular uptake, but still remain primarily associated with the outside of the cell. In MDA-MB-231 cells, fluorescent NDs with and without ProGlo demonstrate primarily punctate fluorescent within cells, indicating that they are likely located in endocytotic vesicles, consistent with uptake by macropinocytosis.