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. 2019 Nov 20;9:17207. doi: 10.1038/s41598-019-53627-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with A_2AR antagonist decreases microglia reactivity in the retina of diabetic mice. (A) Retinal sections were stained with antibodies against Iba-1 (green) and MHC-II (red). Nuclei were stained with DAPI (blue). Representative images are depicted and arrows indicate some MHC-II⁺ Iba-1⁺ cells found in each condition. (B) Activated microglia (MHC-II⁺ Iba-1⁺ cells) were counted and normalized to the percentage of total microglial cells (Iba-1⁺ cells) from 7–9 animals. (C) The number of microglia per retinal section was counted. (D) TSPO protein levels were assessed by Western blot and the results are expressed as a ratio to β-actin from 6–9 independent experiments. Representative Western blots are presented. Full length uncropped images are presented as Supplementary Fig. 1. One-way ANOVA test, followed by Holm-Šídák multiple comparison test. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Scale bar: 100 µm.