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. 2019 Nov 20;9:17207. doi: 10.1038/s41598-019-53627-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Administration of the A_2AR antagonist decreases cell death in the retina of diabetic animals. (A) Cell death was evaluated by TUNEL assay in vertical retinal sections. Nuclei were stained with DAPI (blue). Representative images are depicted (B). The number of TUNEL⁺ cells (white) was counted from 7 to 11 independent experiments. (C) The presence of active caspases in the ganglion cell layer was assessed with CAS-MAP probe (grey). Representative images are depicted. (D) The number of cells CAS-MAP⁺ was counted from 5–9 independent experiments. One-way ANOVA test, followed by Holm-Šídák multiple comparison test. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Scale bar: 100 µm.