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. 2019 Nov 20;9:17207. doi: 10.1038/s41598-019-53627-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A_2AR antagonist attenuates RGC loss in a mouse model of diabetic retinopathy. (A) The number of RGCs was evaluated in vertical sections by immunolabeling against Brn3a (grey). The nuclei were stained with DAPI (blue). Representative images are depicted. (B) The number of Brn3a⁺ cells were counted, and the results were normalized to the length of the section; from 7–13 animals. One-way ANOVA test, followed by Holm-Šídák multiple comparison test. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Scale bar: 100 µm.