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. 2019 Nov 20;9:17179. doi: 10.1038/s41598-019-53135-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vaccinated mice with rSsEno100 and PGA + rSsEno100 showed differences in Th1, Th2 and Th17 cytokine profiles. BALB/c mice were s.c. immunized three times with rSsEno100, PGA + rSsEno100 or PBS as a negative control. Total splenocytes of each animal were obtaining seven days after the last immunization and stimulated in vitro with rSsEno. After 24 h of incubation, supernatant-accumulated cytokines (IL-2, IL-4, IL-6, IL17A, IFN-γ, TNF and IL-10) were measured by cytokine cytometric bead array kit ELISA. The results are presented as the mean ± SD of 5 mice from one of three independent experiments, and statistical significance was determined by one-way ANOVA using Tukey’s multiple comparisons test and a 95% confidence interval. *(p < 0.05), **(p < 0.01), ***(p < 0.001) and ****(p < 0.0001) for comparison the control group or as indicated.