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. 2019 Sep 30;47(21):11250–11267. doi: 10.1093/nar/gkz820

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — PAR-dependent chromatin remodeling leads to an increase in the association rates of DNA-binding domains at sites of DNA damage. (A) Representative image of a nucleus in which the FCS measurements are performed to assess the local dynamics of GFP-BZIP before (pre) and after (post) DNA-damage induction. Microirradiation area is highlighted with green arrowheads. FCS measurement points are shown in blue. Images are pseudocolored according to the look-up table displayed on the right. Scale bar = 4 μm. (B) ratios estimated from FCS curves fitted with an effective diffusion model pre- and ∼1–2 min after (post) DNA damage induction in the presence or absence of PARP inhibitor (PARPi) for GFP-BZIP. (C) ratios estimated from FCS curves fitted with an effective diffusion model pre- and ∼1–2 min after (post) DNA damage induction for GFP-LANA-1–32. (D) Normalized FCS correlation curves obtained for GFP-BZIP in undamaged nuclei. The experimental curve (continuous black line) is fitted with an effective-diffusion model (dotted black line) or a reaction-dominant model (dotted red line). (E and F) Association (k′_on) and dissociation rates (k_off) measured by FCS for GFP-BZIP pre- and post-DNA damage and in untreated cells or cells treated with PARP inhibitor (PARPi).

Inline graphic — PAR-dependent chromatin remodeling leads to an increase in the association rates of DNA-binding domains at sites of DNA damage. (A) Representative image of a nucleus in which the FCS measurements are performed to assess the local dynamics of GFP-BZIP before (pre) and after (post) DNA-damage induction. Microirradiation area is highlighted with green arrowheads. FCS measurement points are shown in blue. Images are pseudocolored according to the look-up table displayed on the right. Scale bar = 4 μm. (B) ratios estimated from FCS curves fitted with an effective diffusion model pre- and ∼1–2 min after (post) DNA damage induction in the presence or absence of PARP inhibitor (PARPi) for GFP-BZIP. (C) ratios estimated from FCS curves fitted with an effective diffusion model pre- and ∼1–2 min after (post) DNA damage induction for GFP-LANA-1–32. (D) Normalized FCS correlation curves obtained for GFP-BZIP in undamaged nuclei. The experimental curve (continuous black line) is fitted with an effective-diffusion model (dotted black line) or a reaction-dominant model (dotted red line). (E and F) Association (k′_on) and dissociation rates (k_off) measured by FCS for GFP-BZIP pre- and post-DNA damage and in untreated cells or cells treated with PARP inhibitor (PARPi).