Fig. 6.

Effects of the timing of the pollination on inbreeding depression (δ) for reproductive performance (A–C) and the delayed self-pollination cost (D–F; see the Materials and Methods) in three populations of D. scandens: Tulum (more outcrossing phenotype), La Mancha (intermediately outcrossing phenotype) and Valladolid (selfing phenotype). Points represent mean inbreeding depression and delayed self-pollination cost at each ontogenetic stage, obtained by non-parametric bootstrapping. Positive values indicate better performance of outbred blossoms than inbred blossoms, and negative values indicate better performance of inbred blossoms than outbred blossoms. Error bars (95 % CIs) overlapping zero indicate values not statistically different from zero. Pollination timings were 1, first day of the female phase; 2, first day of the bisexual phase; 3, last day of the bisexual phase; and 4, during the second female phase.