Figure 7.
S80 phosphorylation regulates transcription in a κB sequence specific manner. WT and NFKB1S80A HEK293T cells were transfected with pTAL-NF-κB reporter constructs with four identical tandem κB-sites that vary at the −1 and −2 positions as indicated (underlined). Twenty four hours post transfection, cells were either left untreated or treated with TNFα for 8 h before luciferase activity was measured. The Renilla luciferase expression vector pRLTK was used as an internal control to normalize transfection efficiency across all samples. Reporter activity is represented as fold increase over untreated WT cells. Data shown are mean ± S.E. of quadruplicate samples. Statistical significance between treated WT and NFKB1S80A cells was determined by Student's t test. **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.