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. 2019 Sep 23;47(21):11225–11237. doi: 10.1093/nar/gkz810

Figure 7.

Figure 7.

BLM cannot unwind dsDNA bound by RAD51. (A) ATPase assays containing 5 nM unlabeled BLM in the presence of 0, 0.5, 1 or 3 μM RAD51 and dsDNA (pUC19). Data points represent the mean and standard deviation of three independent experiments. (B) ATPase assays containing 5 nM GFP–BLM in the presence of 0, 0.5, 1 or 3 μM RAD51 and dsDNA (pUC19). Data points represent the mean and standard deviation of three independent experiments. (C) Schematic illustration of a double-tethered dsDNA curtain coated with RAD51. (D) Kymograph showing that GFP–BLM (0.4 nM) can bind to RAD51-bound dsDNA, but is unable to unwind the dsDNA; note that buffer flow was OFF during data collection, unbound RAD51 was flushed from the sample chamber prior to the injection of GFP–BLM and the reactions contained 1 nM RPA-Cherry.