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. 2019 Sep 23;47(21):11225–11237. doi: 10.1093/nar/gkz810

Figure 8.

Figure 8.

BLM cannot dismantle RAD51-bound heteroduplex DNA joints. (A) Schematic illustration of heteroduplex joints prepared with RAD51–ssDNA curtains and an Atto565-labeled dsDNA oligonucleotide (70-bp) bearing 15-nts of sequence homologous to the M13-derived ssDNA substrate. (B) Percent of GFP–BLM (15 nM) molecules bound the Atto565-labeled heteroduplex DNA joints or bound elsewhere on the RAD51–ssDNA (N = 116). (C) Kymograph showing outcomes of reactions containing GFP–BLM (15 nM) with RAD51–ssDNA curtains preassembled with the Atto565-labeled heteroduplex DNA joints; note that buffer flow was OFF during data collection. The Asterix highlights an example of bound GFP–BLM that did not co-localize with the heteroduplex DNA joint.